In previous posts, we learned how to use Python to detect group differences on a single dependent variable. However, there may be situations in which we are interested in several dependent variables. In these situations, the simple ANOVA model is inadequate.

One way to examine multiple dependent variables using Python would, of course, be to carry out multiple ANOVA. That is, one ANOVA for each of these dependent variables. However, the more tests we conduct on the same data, the more we inflate the family-wise error rate (the greater chance of making a Type I error).

This is where MANOVA comes in handy. MANOVA, or Multivariate Analysis of Variance, is an extension of Analysis of Variance (ANOVA). However, when using MANOVA we have two, or more, dependent variables.

MANOVA and ANOVA is similar when it comes to some of the assumptions. That is, the data have to be:

- normally distributed dependent variables
- equal covariance matrices

In this post will learn how carry out MANOVA using Python (i.e., we will use Pandas and Statsmodels). Here, we are going to use the Iris dataset which can be downloaded here.

## What is MANOVA?

First, we going to have brief introduction to what MANOVA is. MANOVA is the acronym for Multivariate Analysis of Variance. When analyzing data, we may encounter situations where we have there multiple response variables (dependent variables). As mentioned before, by using MANOVA we can test them simultaneously.

### MANOVA Example

Before getting into how to do a MANOVA in Python, let’s look at an example where MANOVA can be a useful statistical method. Assume we have a hypothesis that a new therapy is better than another, more common, therapy (or therapies, for that matter). In this case, we may want to look at the effect of therapies (independent variable) on the mean values of several dependent variables.

For instance, we may be interested in whether the therapies help for a specific psychological disorder (e.g., depression), at the same time as we want to know how it changes life satisfaction, lower suicide risk, among other things. In such an experiment a MANOVA lets us test our hypothesis for all three dependent variables at once.

### Assumptions of MANOVA

In this section, we will briefly discuss some of the assumptions of carrying out MANOVA. There are certain conditions that need to be considered.

- The dependent variables should be normally distributed within groups. That is, in the example below the dependent variables should be normally distributed within the different treatment groups.
- Homogeneity of variances across the range of predictors. This assumption can be tested in Python using Bartlett’s test or Levene’s test.
- Linearity between all pairs of dependent variables (e.g., between depression, life satisfaction, and suicide risk), all pairs of covariates, and all dependent variable-covariate pairs in each cell

Now, if you only have one dependent variable, two groups, and your data is not following the normal distribution there is still hope. You can, in fact, carry out non-parametric statistical tests such as the Mann-Whitney U test in Python, as well.

## Prerequisites

To carry out MANOVA in Python you need to have the package statsmodels installed. Here you will learn how to install this Python package.

### Installing Statsmodels

We can install Python packages with either conda or pip, for example. Here’s how to install statsmodels using pip:

Code language: Bash (bash)`pip install statsmodels`

Typicall, it is considered good practice to install Python packages in virtual environments. Check this YouTube video for more information on how to install statsmodels in a virtual environment (both with pip and conda):

Note, we will also use Pandas to read a csv file but installing statsmodels will also install Pandas. In the next section, we will learn how to perform MANOVA using statsmodels.

## How to Carry out MANOVA in Python

In this section, we will focus on how to conduct the Python MANOVA using Statsmodels. First, the first code example, below, we are going to import Pandas as pd. Second, we import the MANOVA class from statsmodels.multivariate.manova.

```
import pandas as pd
from statsmodels.multivariate.manova import MANOVA
```

Code language: Python (python)

Before carrying out the Python MANOVA we need some example data. This is why we use Pandas. In the next code chunk, we are going to read a CSV file from a URL using Pandas read_csv. We are also going to replace the dots (“.”) in the column names with underscores (“_”). If you need to find out more about cleaning your data see post *data cleaning in Python with Pandas*.

```
url = 'https://vincentarelbundock.github.io/Rdatasets/csv/datasets/iris.csv'
df = pd.read_csv(url, index_col=0)
df.columns = df.columns.str.replace(".", "_")
df.head()
```

Code language: Python (python)

Learn more about working with Pandas dataframe:

- Pandas Read Excel Tutorial
- Read CSV files using Pandas
- How to Group Data using Pandas Groupby
- Pandas Dataframe Tutorial

### Python MANOVA Example

Now that we have read a data file (i.e., a CSV file) using Pandas we are ready to carry out the MANOVA in Python. In the Python MANOVA example below we are going to use the from_formula method. This method takes the formula as a string object.

In this MANOVA example, we are going to use the width and length columns as dependent variables. Furthermore, the species column is used as independent variable. That is, we are carrying out a one-way MANOVA here:

```
maov = MANOVA.from_formula('Sepal_Length + Sepal_Width + \
Petal_Length + Petal_Width ~ Species', data=df)
```

Code language: Python (python)

Finally, we have used Python to do a one-way MANOVA. The last thing to do is to print the MANOVA table using the *mv_test *method:

Code language: Python (python)`print(maov.mv_test())`

Here’s a link to a Jupyter Notebook containing the MANOVA Statsmodels example in this post. Make sure you check the recent post, How to Perform a Two-Sample T-test with Python: 3 Different Methods, for a recent Python data analysis tutorial.

## Conclusion

In this post, we learned how to carry out a Multivariate Analysis of Variance (MANOVA) using Python and Statsmodels. More specifically we have:

- used Pandas do load a dataset from a CSV file.
- cleaned column names of a Pandas dataframe
- learned multivariate analysis by a MANOVA statsmodels example